Genome-scale DNA methylation mapping of clinical samples at single-nucleotide resolution.

Nat Methods
Authors
Keywords
Abstract

Bisulfite sequencing measures absolute levels of DNA methylation at single-nucleotide resolution, providing a robust platform for molecular diagnostics. We optimized bisulfite sequencing for genome-scale analysis of clinical samples: here we outline how restriction digestion targets bisulfite sequencing to hotspots of epigenetic regulation and describe a statistical method for assessing significance of altered DNA methylation patterns. Thirty nanograms of DNA was sufficient for genome-scale analysis and our protocol worked well on formalin-fixed, paraffin-embedded samples.

Year of Publication
2010
Journal
Nat Methods
Volume
7
Issue
2
Pages
133-6
Date Published
2010 Feb
ISSN
1548-7105
URL
DOI
10.1038/nmeth.1414
PubMed ID
20062050
PubMed Central ID
PMC2860480
Links
Grant list
R01 HG004401 / HG / NHGRI NIH HHS / United States
U54 HG003067 / HG / NHGRI NIH HHS / United States
R01 HG004401-03 / HG / NHGRI NIH HHS / United States
U01ES017155 / ES / NIEHS NIH HHS / United States
R01HG004401 / HG / NHGRI NIH HHS / United States
U54HG03067 / HG / NHGRI NIH HHS / United States
U01 ES017155-02 / ES / NIEHS NIH HHS / United States
U01 ES017155 / ES / NIEHS NIH HHS / United States
U54 HG003067-08 / HG / NHGRI NIH HHS / United States