Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts.

Genome Biol
Authors
Keywords
Abstract

Targeted RNA-Seq combines next-generation sequencing with capture of sequences from a relevant subset of a transcriptome. When testing by capturing sequences from a tumor cDNA library by hybridization to oligonucleotide probes specific for 467 cancer-related genes, this method showed high selectivity, improved mutation detection enabling discovery of novel chimeric transcripts, and provided RNA expression data. Thus, targeted RNA-Seq produces an enhanced view of the molecular state of a set of "high interest" genes.

Year of Publication
2009
Journal
Genome Biol
Volume
10
Issue
10
Pages
R115
Date Published
2009
ISSN
1474-760X
URL
DOI
10.1186/gb-2009-10-10-r115
PubMed ID
19835606
PubMed Central ID
PMC2784330
Links
Grant list
DP2OD002750 / OD / NIH HHS / United States
HG03067-05 / HG / NHGRI NIH HHS / United States