Introduction: cell-based assays for high-throughput screening.

Methods Mol Biol
Authors
Keywords
Abstract

Cell-based assays represent approximately half of all high-throughput screens (HTS) currently performed. Here we review the history and status of HTS, and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS, drawing upon themes that will reemerge in subsequent chapters in this book. Approaches for successful experimental design and execution of cell-based HTS are introduced, including strategies for assay development, implementation of primary and secondary screens, and target identification. In doing so, we hope to provide a comprehensive review of the cell-based HTS process and an introduction to the methodologies and techniques described in this book.

Year of Publication
2009
Journal
Methods Mol Biol
Volume
486
Pages
1-12
Date Published
2009
ISSN
1064-3745
URL
DOI
10.1007/978-1-60327-545-3_1
PubMed ID
19347612
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