Complex phenotypic assays in high-throughput screening.

Curr Opin Chem Biol
Authors
Keywords
Abstract

High-throughput screening (HTS), systematically testing thousands of small molecules to find candidates for lead optimization, primarily involves exposure of purified proteins to arrayed collections of small molecules. More complex phenotypic assays, such as cell-based or whole-organism assays, traditionally have flanked HTS, preceding it to validate new therapeutic targets, and following it to characterize new lead compounds in cellular contexts. Recently, however, cell- and organism-based phenotypic assays have increasingly been adopted as a primary screening platform for annotating small molecules.

Year of Publication
2004
Journal
Curr Opin Chem Biol
Volume
8
Issue
3
Pages
334-8
Date Published
2004 Jun
ISSN
1367-5931
URL
DOI
10.1016/j.cbpa.2004.04.002
PubMed ID
15183333
Links