Activity-dependent A-to-I RNA editing in rat cortical neurons.
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Abstract | Changes in neural activity influence synaptic plasticity/scaling, gene expression, and epigenetic modifications. We present the first evidence that short-term and persistent changes in neural activity can alter adenosine-to-inosine (A-to-I) RNA editing, a post-transcriptional site-specific modification found in several neuron-specific transcripts. In rat cortical neuron cultures, activity-dependent changes in A-to-I RNA editing in coding exons are present after 6 hr of high potassium depolarization but not after 1 hr and require calcium entry into neurons. When treatments are extended from hours to days, we observe a negative feedback phenomenon: Chronic depolarization increases editing at many sites and chronic silencing decreases editing. We present several different modulations of neural activity that change the expression of different mRNA isoforms through editing. |
Year of Publication | 2012
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Journal | Genetics
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Volume | 192
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Issue | 1
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Pages | 281-7
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Date Published | 2012 Sep
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ISSN | 1943-2631
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URL | |
DOI | 10.1534/genetics.112.141200
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PubMed ID | 22714409
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PubMed Central ID | PMC3430542
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Grant list | Howard Hughes Medical Institute / United States
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