Integrated proteomic analysis of post-translational modifications by serial enrichment.

Nat Methods
Authors
Keywords
Abstract

We report a mass spectrometry-based method for the integrated analysis of protein expression, phosphorylation, ubiquitination and acetylation by serial enrichments of different post-translational modifications (SEPTM) from the same biological sample. This technology enabled quantitative analysis of nearly 8,000 proteins and more than 20,000 phosphorylation, 15,000 ubiquitination and 3,000 acetylation sites per experiment, generating a holistic view of cellular signal transduction pathways as exemplified by analysis of bortezomib-treated human leukemia cells.

Year of Publication
2013
Journal
Nat Methods
Volume
10
Issue
7
Pages
634-7
Date Published
2013 Jul
ISSN
1548-7105
URL
DOI
10.1038/nmeth.2518
PubMed ID
23749302
PubMed Central ID
PMC3943163
Links
Grant list
U24 CA160034 / CA / NCI NIH HHS / United States
R01HL096738 / HL / NHLBI NIH HHS / United States
R01 HL096738 / HL / NHLBI NIH HHS / United States
HHSN268201000033C / PHS HHS / United States
HHSN268201000033C / HL / NHLBI NIH HHS / United States
U24CA160034 / CA / NCI NIH HHS / United States