Integrated proteomic analysis of post-translational modifications by serial enrichment.
Nat Methods
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Abstract | We report a mass spectrometry-based method for the integrated analysis of protein expression, phosphorylation, ubiquitination and acetylation by serial enrichments of different post-translational modifications (SEPTM) from the same biological sample. This technology enabled quantitative analysis of nearly 8,000 proteins and more than 20,000 phosphorylation, 15,000 ubiquitination and 3,000 acetylation sites per experiment, generating a holistic view of cellular signal transduction pathways as exemplified by analysis of bortezomib-treated human leukemia cells. |
Year of Publication | 2013
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Journal | Nat Methods
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Volume | 10
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Issue | 7
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Pages | 634-7
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Date Published | 2013 Jul
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ISSN | 1548-7105
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URL | |
DOI | 10.1038/nmeth.2518
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PubMed ID | 23749302
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PubMed Central ID | PMC3943163
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Grant list | U24 CA160034 / CA / NCI NIH HHS / United States
R01HL096738 / HL / NHLBI NIH HHS / United States
R01 HL096738 / HL / NHLBI NIH HHS / United States
HHSN268201000033C / PHS HHS / United States
HHSN268201000033C / HL / NHLBI NIH HHS / United States
U24CA160034 / CA / NCI NIH HHS / United States
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