RNA-guided genome editing of mammalian cells.

Methods Mol Biol
Authors
Keywords
Abstract

The microbial CRISPR-Cas adaptive immune system can be harnessed to facilitate genome editing in eukaryotic cells (Cong L et al., Science 339, 819-823, 2013; Mali P et al., Science 339, 823-826, 2013). Here we describe a protocol for the use of the RNA-guided Cas9 nuclease from the Streptococcus pyogenes type II CRISPR system to achieve specific, scalable, and cost-efficient genome editing in mammalian cells.

Year of Publication
2014
Journal
Methods Mol Biol
Volume
1114
Pages
269-77
Date Published
2014
ISSN
1940-6029
URL
DOI
10.1007/978-1-62703-761-7_17
PubMed ID
24557909
Links
Grant list
DP1-MH100706 / DP / NCCDPHP CDC HHS / United States
R01-NS073124 / NS / NINDS NIH HHS / United States