Reproducibility of metabolomic profiles among men and women in 2 large cohort studies.

Clin Chem
Authors
Keywords
Abstract

BACKGROUND: Rigorous studies are necessary to demonstrate suitability of metabolomics platforms to profile metabolites in archived plasma within epidemiologic studies of human disease, for which attenuation of effect estimates due to measurement error is a key concern.

METHODS: Using a liquid chromatography-tandem mass spectrometry platform, we quantified 257 metabolites from archived plasma to evaluate metabolite interassay reproducibility, reproducibility with delayed processing, and within-person reproducibility over time. Interassay reproducibility was assessed with CVs from 60 duplicate plasma samples donated by participants in the Nurses' Health Study and Health Professionals Follow-up Study, and 20 QC pool plasma replicates. Metabolite reproducibility over a 24- to 48-h processing delay (n = 48 samples) and within-person reproducibility over 1-2 years (n = 80 samples) were assessed using Spearman and intraclass correlation coefficients (ICCs).

RESULTS: CVs were

CONCLUSIONS: For potential use in epidemiologic studies, the majority of plasma metabolites had low CVs and were reproducible over a 24-h processing delay and within individuals over 1-2 years. Certain metabolites, such as carbohydrates and purine/pyrimidine derivatives, may be challenging to evaluate if samples have delayed processing.

Year of Publication
2013
Journal
Clin Chem
Volume
59
Issue
11
Pages
1657-67
Date Published
2013 Nov
ISSN
1530-8561
URL
DOI
10.1373/clinchem.2012.199133
PubMed ID
23897902
PubMed Central ID
PMC3812240
Links
Grant list
P01 CA087969 / CA / NCI NIH HHS / United States
P01 CA055075 / CA / NCI NIH HHS / United States
K07-CA140790 / CA / NCI NIH HHS / United States
Howard Hughes Medical Institute / United States
R01-CA49449 / CA / NCI NIH HHS / United States
K07 CA140790 / CA / NCI NIH HHS / United States
P01-CA87969 / CA / NCI NIH HHS / United States
R01 CA049449 / CA / NCI NIH HHS / United States
P01-CA055075 / CA / NCI NIH HHS / United States