Targeted metabolomics.

Curr Protoc Mol Biol
Authors
Keywords
Abstract

The metabolome is the terminal downstream product of the genome and consists of the total complement of all the low-molecular-weight molecules (metabolites) in a cell, tissue, or organism. Metabolomics aims to measure a wide breadth of small molecules in the context of physiological stimuli or disease states. Metabolomics methodologies fall into two distinct groups: untargeted metabolomics, an intended comprehensive analysis of all the measurable analytes in a sample including chemical unknowns, and targeted metabolomics, the measurement of defined groups of chemically characterized and biochemically annotated metabolites. The methodologies considered in this unit focus on the processes of conducting targeted metabolomics experiments, and the advantages of this general approach are highlighted herein. This unit outlines procedures for extracting nitrogenous metabolites (including amino acids), lipids, and intermediary metabolites (including TCA cycle oxoacids) from blood plasma. Specifically, protocols are described for analyzing these metabolites using targeted metabolomics experiments based on liquid chromatography-mass spectrometry.

Year of Publication
2012
Journal
Curr Protoc Mol Biol
Volume
Chapter 30
Pages
Unit 30.2.1-24
Date Published
2012 Apr
ISSN
1934-3647
URL
DOI
10.1002/0471142727.mb3002s98
PubMed ID
22470063
PubMed Central ID
PMC3334318
Links
Grant list
N01-HC-25195 / HC / NHLBI NIH HHS / United States
R01-DK081572 / DK / NIDDK NIH HHS / United States
R01 DK081572 / DK / NIDDK NIH HHS / United States
R01 DK081572-02 / DK / NIDDK NIH HHS / United States
R01 HL098280 / HL / NHLBI NIH HHS / United States
R01 HL098280-03 / HL / NHLBI NIH HHS / United States
R01-HL098280 / HL / NHLBI NIH HHS / United States
R01-HL096738 / HL / NHLBI NIH HHS / United States