Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning.

Small
Authors
Keywords
Abstract

Living-cell-based screens can facilitate lead discovery of functional therapeutics of interest. A versatile and scalable method is reported that uses dense arrays of nanowells for imparting defined patterns on monolayers of cells. It is shown that this approach can coordinate a multi-component biological assay by designing and implementing a high-throughput, functional nanoliter-scale neutralization assay to identify neutralizing antibodies against HIV.

Year of Publication
2015
Journal
Small
Volume
11
Issue
36
Pages
4643-50
Date Published
2015 Sep
ISSN
1613-6829
URL
DOI
10.1002/smll.201500674
PubMed ID
26121321
PubMed Central ID
PMC4754792
Links
Grant list
P30 CA014051 / CA / NCI NIH HHS / United States
U19 AI090970 / AI / NIAID NIH HHS / United States
5 U19 A1090970 / PHS HHS / United States
P30-CA14051 / CA / NCI NIH HHS / United States